Antimicrobial Activity Test of Key Pepet Extract (Kaempferia Rotunda L.)

Abstract

Kunci Pepet Rhizome is a medicinal plant  from  ginger family which contains many secondary metabolites including essential oils, flavonoids  and  terpenoids, which can act as therapy for dysentery, diarrhea, and also for body slim treatment. The extraction method that was used in this research is fractionated solvent extraction using n-hexane, dichloromethane and ethanol. Those fractions were tested for its antimicrobial activities using agar diffusion method. Identification of the qualitative  content of secondary metabolites is carried out by TLC. Inhibition zones of ethanol extract, n-hexane, dichloromethane and aqueous fraction against Escherichia coli, Staphylococcus aureus and Candida albicans were 2.08cm, 1.37cm, 1.47cm, 1.27cm, 2.13cm, 1.30cm, 1.30cm, 1.57cm, 1.82cm, 1.27cm, 1.50cm, 1.33cm, respectively. The inhibition zones of the crude extract, fractions of n-hexane, dichloromethane and aqueous fraction are 1.82cm, 1,27cm, 1.50cm, 1.33cm. GCMS analysis of the extract showed that it contains contains benzoic acid (8.97%), hexadecane (28.84% and 33.50%), benzyl benzoate (89.21%, 14.71%, 7.23% and 19.88%), 2-hydroxy benzoic acid ester phenilmethil (0.90%), heksadekanoat  acid (0.92%, 10.10%, 7.04% and 11.71%), benzyl alcohol (12.30%), pentadekana (7.22%), octadecanoic methyl ester (5.43%).